Fig. 3

Characterization of olive oil consumption by ZS6. a Gravimetric analysis of oil assimilation by ZS6. X- and Y-axes indicate the time (h) and percent of cell mass (closed circle) and oil mass (open circle). b Thin-layer chromatographic analysis of oil residues extracted from supernatant of ZS6 cultures at various time points indicated. Equal amount of hexane extracts is loaded on TLC plate. Separated fractions of compounds are deduced based on their molecular weight determined using MS analysis. TAG triacylglycerol, FFA free fatty acid, DAG diacylglycerol, MAG monoacylglycerol. c Changes of lipid compositions in culture. Total lipids are mainly consistent of TAG (open rectangle), FFA (stripped rectangle), DAG and MAG (black rectangle). d ZS6 lipase activity in MS medium supplemented with olive oil and YE. X- and Y- axes indicate time (h) and lipase activity (U L⁻¹). Closed and open circles indicate cells in medium supplemented with olive oil (OO) and YE, respectively. e Efficiency of lipase production per unit of cell mass. Ratio of maximum lipase activity in U and CDW. f Productivity of lipase activity in U per hour per liter