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Table 4 Milk clotting and proteolytic activity of purified MCAP and its mutants

From: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Sample MCA (U µg−1) Proteolytic activity PA (U µg−1) Ratio MCA/PA
MCAP-3 (non-mutated) 72 4.26 ± 0.82 17.35 ± 3.75
SyMCAP-6 (synthetic) 137 7.02 ± 0.28 19.53 ± 0.79
MutMCAP-8 (mutated) 204 13.56 ± 1.47 15.13 ± 1.64
M. mihei 311 11.11 ± 0.27 28 ± 0.68a
  1. Results shown are the means of three sets of experiments
  2. aData from Gama Salgado et al. (2013)