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Table 4 Milk clotting and proteolytic activity of purified MCAP and its mutants

From: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Sample

MCA (U µg−1)

Proteolytic activity PA (U µg−1)

Ratio MCA/PA

MCAP-3 (non-mutated)

72

4.26 ± 0.82

17.35 ± 3.75

SyMCAP-6 (synthetic)

137

7.02 ± 0.28

19.53 ± 0.79

MutMCAP-8 (mutated)

204

13.56 ± 1.47

15.13 ± 1.64

M. mihei

311

11.11 ± 0.27

28 ± 0.68a

  1. Results shown are the means of three sets of experiments
  2. aData from Gama Salgado et al. (2013)
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