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Table 3 Summary of production and purification of MCAP

From: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Sample Step Volume (mL) Proteina (mg) Recovery (%) Purification (fold)
MCAP-3 (non-mutated) Crude 49 8.44 100 1
IEX 12.5 4.67 55.3 2.1
SyMCAP-6 (synthetic) Crude 50 14 100 1
IEX 12.5 9.17 65.5 1.5
MutMCAP-8 (mutated) Crude 48 5.76 100 1
IEX 7.5 3.5 60.7 6.6
  1. aDate are given as average, n = 3