Table 3 Summary of production and purification of MCAP
Sample | Step | Volume (mL) | Proteina (mg) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|
MCAP-3 (non-mutated) | Crude | 49 | 8.44 | 100 | 1 |
IEX | 12.5 | 4.67 | 55.3 | 2.1 | |
SyMCAP-6 (synthetic) | Crude | 50 | 14 | 100 | 1 |
IEX | 12.5 | 9.17 | 65.5 | 1.5 | |
MutMCAP-8 (mutated) | Crude | 48 | 5.76 | 100 | 1 |
IEX | 7.5 | 3.5 | 60.7 | 6.6 |