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Fig. 1 | AMB Express

Fig. 1

From: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Fig. 1

Recombinant plasmid construction pGAM1 + MCAP-3: a map of pGAM1 + MCAP-3, b diagram of the MCAP gene showing the Kozak consensus located between the GAP promoter and the SP MCAP sequence of pGAM1 + MCAP-3. c The identity of the signal peptide sequence (SP MCAP) of M. circinelloides with other fungal counterparts. The residues are numbered according to the signal sequence cleavage (between position − 1 and 1: A-AP). The residues identical are shaded in grey

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