Table 1 The primer sequences used in this study
Mutated site | Primer sequences |
|---|---|
SS-tan S210A | F: GTCTCTGCAGGTACCGCTGCGGGTGGC |
R: AGCGGTACCTGCAGAGACAATACGTTCAAC | |
SS-tan K371A | F: GTTGGTGCACGTAAAGCAACGACCCCG |
R: TGCTTTACGTGCACCAACGTGCGTCAGG | |
SS-tan E385A | F: GACCTGTCTACCGGTGCTAACAACCTGTTTG |
R: AGCACCGGTAGACAGGTCGAAAGCATC | |
SS-tan D453A | F: CGTCTGGGTACCAACGCTACGGACACCT |
R: AGCGTTGGTACCCAGACGAATCCAC | |
SS-tan D455A | F: GGTACCAACGATACGGCTACCTCCCACGT |
R: AGCCGTATCGTTGGTACCCAGACGAAT | |
SS-tan H485A | F: TACTGGGACCAGGGTGCTGGCGCCAAT |
R: AGCACCCTGGTCCCAGTAATAC | |
SS-tan G384P | F: CTTTCGACCTGTCTACCCCAGAAAACAACCTGTTTG |
R:CAAACAGGTTGTTTTCTGGGGTAGACAGGTCGAAAG | |
LP-tan P356G | F:CCAATTAGATTTGACGAGTGGTGAGAATAATTTATTTGGCG |
R:CGCCAAATAAATTATTCTCACCACTCGTCAAATCTAATTGG |