The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains

AMB Express

Table 1 Primers used for the PCR amplification of the hsp70 gene of Streptomyces strains

Primer	Oligonucleotide sequence^a	Length (bases)	Positions^b	Orientation
hsp70-U7F	CGTGCAGTCGGTATCGACCTCGGBACVACBAACTC	35	7–41	Sense
hsp70-1326R	CGATGCCGTTGGCGTCGATGTCGAASGHSACCTCGA	36	1326–1363	Antisense
U1F^c	CGTGCAGTCGGTATCGACCTCGG	23	7–29	Sense
2R^c	CGATGCCGTTGGCGTCGATGTC	22	1326–1347	Antisense

^aIUB code for degenerated base positions: B = G/C/T; V = A/G/C; S = C/G; H = A/C/T
^bPositions correspond to the hsp70(DnaK) nucleotide sequence of S. coelicolor (GeneBank NO. L46700)
^cDirect sequencing primers for hsp70-U7F/hsp70-1326R