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Table 4 Experimental design of steepest ascent and corresponding results

From: Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method

Run

Shaking speed (rmp)

Culture temperature (°C)

pH

Total viable B. mucilaginous (107 Obj/mL)

1

250

24

6.0

1.06 ± 0.01

2

200

28

6.5

2.17 ± 0.02

3

150

32

7.0

1.92 ± 0.03

4

100

36

7.5

1.05 ± 0.01

5

50

40

8.0

0.62 ± 0.02

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