Table 2 Box–Behnken design matrix
Run | T | DO | IPTG | rPDT band intensity |
|---|---|---|---|---|
1 | 20 | 10 | 112.5 | 10934.5 |
2 | 40 | 10 | 112.5 | 6874.91 |
3 | 20 | 50 | 112.5 | 11531.7 |
4 | 40 | 50 | 112.5 | 6147.456 |
5 | 20 | 30 | 25 | 10745.06 |
6 | 40 | 30 | 25 | 5949.517 |
7 | 20 | 30 | 200 | 11619.7 |
8 | 40 | 30 | 200 | 6395.547 |
9 | 30 | 10 | 25 | 8968.991 |
10 | 30 | 50 | 25 | 9389.53 |
11 | 30 | 10 | 200 | 9104.195 |
12 | 30 | 50 | 200 | 9437.547 |
13 | 30 | 30 | 112.5 | 9953.16 |
14 | 30 | 30 | 112.5 | 10302.3 |
15 | 30 | 30 | 112.5 | 10235 |