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Table 1 Coded and real values for the Box–Behnken design

From: Optimization of rPDT fusion protein expression by Escherichia coli in pilot scale fermentation: a statistical experimental design approach

Variable Variable code − 1 0 + 1
Temperature (°C) X1 20 30 40
DO (%) X2 10 30 50
IPTG (μM) X3 25 112.5 200