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Table 1 Coded and real values for the Box–Behnken design

From: Optimization of rPDT fusion protein expression by Escherichia coli in pilot scale fermentation: a statistical experimental design approach

Variable

Variable code

− 1

0

+ 1

Temperature (°C)

X1

20

30

40

DO (%)

X2

10

30

50

IPTG (μM)

X3

25

112.5

200

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