Glucose limited feed strategy leads to increased production of fusicocca-2,10(14)-diene by Saccharomyces cerevisiae

Halka, Lisa Marie; Nowacki, Christian; Kleinschmidt, Alica; Koenen, Kevin; Wichmann, Rolf

doi:10.1186/s13568-018-0662-8

AMB Express

Table 1 Impact of development of a glucose feed strategy on growth of S. cerevisiae and FCdiene production

From: Glucose limited feed strategy leads to increased production of fusicocca-2,10(14)-diene by Saccharomyces cerevisiae

Fermentations	Name	F1	F2	F3	F4	F5	F6	First fed-batch fermentation
Fermentations	Description	Batch fermentation	Fed-batch fermentation with manual feed	Fed-batch fermentation with controlled feed and variable stirrer speed	Fed-batch fermentation with controlled feed and staircase-shaped stirrer profile	Fed-batch fermentation with controlled feed and linear stirrer profile	Optimized fed-batch fermentation	First fed-batch fermentation
Reference		This study						Arens et al. (2014)
Initial glucose concentration (g/L)		30	1	0	0	0	0	20
Total amount of glucose (g)		52.42	21.53	44.22	74.82	62.26	45.82	nd
Type of feed		None	Pulses based on DOT	Continuous controlled feed	Continuous controlled feed	Continuous controlled feed	Continuous controlled feed	Once 15 g/L
Time interval of const. feedrate (min)		–	–	30	15	15	15	–
Preset µ		–	–	0.8 _max	Variable for each interval	Variable for each interval	Variable for each interval	–
Fermentation time (h)		28	48	48	50	40	48	120
pH		Unregulated	Unregulated	Unregulated	Unregulated	Unregulated	Unregulated	20 mM succinate
Final biomass (g/L)		2.84	5.00	5.50	8.68	8.73	8.34	3.3
Final FCdiene (mg/L)		51.71	256.42	201.73	405.80	249.21	250.46	~ 20
Max. glucose (g/L)		30.75	0.96	3.02	1.99	0.94	0.26	20
Max. ethanol (g/L)		9.67	1.64	6.38	7.15	6.25	3.00	nd
Y_X/S (g_CDW/g_Glc)^a		0.09	0.36	0.22	0.23	0.25	0.32	nd
q_Glc,max (g_Glc/(g_CDW h))		2.50	0.65	0.57	0.50	0.32	0.31	nd
µ_max (1/h)		0.30	0.22	0.27	0.27	0.20	0.15	nd
Y_P/S (mg_FCdiene/g_Glc)^a		1.78	18.82	8.44	10.74	7.34	9.77	nd
Y_P/X (mg_FCdiene/g_CDW)^a		18.95	52.79	37.56	45.94	29.13	30.77	nd
r_FCdiene,X (mg_FCdiene/(g_CDW h))^b		1.27	4.02	1.25	1.43	2.79	1.26	nd
STY (mg_FCdiene/(L h))		1.85	5.34	4.20	9.92	6.23	5.22	nd

nd not determined
Italic values indicates the main changes in the fermentations
^aYields and STY were determined at the end of the fermentations
^bProduct formation rates were determined during exponential growth phase

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