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Table 1 Impact of development of a glucose feed strategy on growth of S. cerevisiae and FCdiene production

From: Glucose limited feed strategy leads to increased production of fusicocca-2,10(14)-diene by Saccharomyces cerevisiae

Fermentations Name F1 F2 F3 F4 F5 F6 First fed-batch fermentation
Description Batch fermentation Fed-batch fermentation with manual feed Fed-batch fermentation with controlled feed and variable stirrer speed Fed-batch fermentation with controlled feed and staircase-shaped stirrer profile Fed-batch fermentation with controlled feed and linear stirrer profile Optimized fed-batch fermentation
Reference This study Arens et al. (2014)
Initial glucose concentration (g/L) 30 1 0 0 0 0 20
Total amount of glucose (g) 52.42 21.53 44.22 74.82 62.26 45.82 nd
Type of feed None Pulses based on DOT Continuous controlled feed Continuous controlled feed Continuous controlled feed Continuous controlled feed Once 15 g/L
Time interval of const. feedrate (min) 30 15 15 15
Preset µ 0.8 max Variable for each interval Variable for each interval Variable for each interval
Fermentation time (h) 28 48 48 50 40 48 120
pH Unregulated Unregulated Unregulated Unregulated Unregulated Unregulated 20 mM succinate
Final biomass (g/L) 2.84 5.00 5.50 8.68 8.73 8.34 3.3
Final FCdiene (mg/L) 51.71 256.42 201.73 405.80 249.21 250.46 ~ 20
Max. glucose (g/L) 30.75 0.96 3.02 1.99 0.94 0.26 20
Max. ethanol (g/L) 9.67 1.64 6.38 7.15 6.25 3.00 nd
YX/S (gCDW/gGlc)a 0.09 0.36 0.22 0.23 0.25 0.32 nd
qGlc,max (gGlc/(gCDW h)) 2.50 0.65 0.57 0.50 0.32 0.31 nd
µmax (1/h) 0.30 0.22 0.27 0.27 0.20 0.15 nd
YP/S (mgFCdiene/gGlc)a 1.78 18.82 8.44 10.74 7.34 9.77 nd
YP/X (mgFCdiene/gCDW)a 18.95 52.79 37.56 45.94 29.13 30.77 nd
rFCdiene,X (mgFCdiene/(gCDW h))b 1.27 4.02 1.25 1.43 2.79 1.26 nd
STY (mgFCdiene/(L h)) 1.85 5.34 4.20 9.92 6.23 5.22 nd
  1. nd not determined
  2. Italic values indicates the main changes in the fermentations
  3. aYields and STY were determined at the end of the fermentations
  4. bProduct formation rates were determined during exponential growth phase
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