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Table 3 Experimental design and responses after lyophilization

From: Statistical modelling for optimized lyophilization of Lactobacillus acidophilus strains for improved viability and stability using response surface methodology

Run

Factors

Responses

% (g/100 mL)

%

Log10 CFU/mL

S

SM

Y1

Y2

Y3

Y4

Y5

Y6

Y7

1

0.00

0.00

50.03

5.00

3.59

2.58

1.58

1.57

1.56

2

6.00

0.00

62.61

11.77

10.70

10.67

10.64

10.59

10.55

3

0.00

6.00

67.63

11.78

10.70

10.68

10.66

10.60

10.56

4

6.00

6.00

76.19

10.79

9.09

9.06

9.00

8.77

8.72

5

− 1.24a

3.00

60.21

8.56

7.56

7.53

7.49

7.42

7.39

6

7.24

3.00

68.83

11.05

10.34

10.33

10.30

10.26

10.20

7

3.00

− 1.24a

57.62

8.54

7.75

7.52

7.46

7.41

7.37

8

3.00

7.24

74.86

11.33

10.40

10.34

10.32

10.30

10.23

9

3.00

3.00

65.96

10.06

9.19

9.05

8.52

8.50

8.48

10

3.00

3.00

65.73

10.06

9.19

9.05

8.52

8.50

8.48

11

3.00

3.00

65.97

10.06

9.19

9.05

8.52

8.50

8.48

12

3.00

3.00

65.72

10.06

9.19

9.05

8.52

8.50

8.48

13

3.00

3.00

65.45

10.06

9.19

9.05

8.52

8.50

8.48

  1. aAs negative concentration cannot be achieved 0 was considered