Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete

AMB Express

Table 1 Isolation and purification of Il-DyP4 and Il-MnP6 from inclusion bodies

	Sample	Protein concentration (mg mL⁻¹)	Protein (mg)	Specific activity (U mg⁻¹)	Total activity (U)	Yield (%)
DyP	Inclusion body	8.78	87.8
	Dialyzed	0.24	28.2	10.8	305	100
	Ni–NTA	0.0093	1.87	140	261	86
MnP	Inclusion body	4.45	22.2
	Dialyzed	0.23	8.1	2	16.2	100
	Ni–NTA	0.0082	0.41	23.8	9.8	60