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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins

Fig. 4

Analysis of sortase activity on LO-Mt-Ch chimeric protein. M: molecular weight marker. Lane 1: recombinant srtA, synthesized in this study. Lane 2: the chimeric protein was partially processed into two fragments of 16 (Mt-Ch) and 14 kDa (Lpp′-ompA) after treatment by sortase (In vitro sortase assay). Lanes 3 and 4: the chimeric protein with out treatment with sortase

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