Genome-scale metabolic model analysis indicates low energy production efficiency in marine ammonia-oxidizing archaea

AMB Express

Table 4 Experimentally measured and computationally calculated yield values for strain SCM1 and other ammonia-oxidizing organisms

Strains	Original experimental data	Biomass/NH₄⁺ yield (gDW mol⁻¹)	Biomass/NH₄⁺ yield (mol mol⁻¹)^c	ATP/Biomass yield (mol g⁻¹)	ATP/NH₄⁺ yield (mol mol⁻¹)	References
Nitrosopumilus maritimus SCM1	3.49 × 10¹³ cells mol⁻¹ NH₄⁺	0.698	1:37	0.213^d	0.149	Löscher et al. (2012)
Nitrosopumilus maritimus SCM1	5 × 10¹³ cells mol⁻¹ NH₄⁺	1.00^a	1:26	0.213^d	0.213	Konneke et al. (2005)
Nitrosopumilus maritimus SCM1	1.3 gDW mol⁻¹ NH₄⁺	1.3	1:20	0.213^d	0.276	Konneke et al. (2014)
Nitrosopumilus maritimus SCM1	0.027 h⁻¹/26.5 mmol NH₄⁺ g DW⁻¹ h⁻¹	1.02	1:25	0.213^d	0.217	Martens-Habbena et al. (2009)
Strian PS0	5.31 × 10¹³ cells mol⁻¹ NH₄⁺	1.06^a	1:25	0.213^d	0.226	Qin et al. (2014)
Strian HCA1	5.50 × 10¹³ cells mol⁻¹ NH₄⁺	1.10^a	1:24	0.213^d	0.234	Qin et al. (2014)
Nitrosopumilus piranensis D3C, Nitrosopumilus adriaticus NF5	6 × 10¹³ cells mol⁻¹ NH₄⁺	1.20^a	1:22	0.213^d	0.256	Bayer et al. (2016)
Nitrosomonas europaea ATCC 19718	0.171 ATP/NH₄⁺ (6% proton circulating in the chain)	0.718^b	1:36	0.238^e	0.171	Poughon et al. (2001)

^a20 fg dry weight cell⁻¹ for strain SCM1 was used to convert the cell abundance figures to dry cell weights. This value was also used in the calculation for strains PS0 HCA1 D3C and NF5 due to their similar cell shapes and sizes (Qin et al. 2014)
^b194 fg dry weight cell⁻¹ for N. europaea was used to convert the cell numbers to dry cell weights. The value for N. europaea was calculated based on the reported values of 120 fg protein dry weight cell⁻¹ and 62% protein content (Martens-Habbena et al. 2009; Poughon et al. 2001)
^cCalculated from the biomass/NH₄⁺ yield based on a molecular weight of 26 g mol⁻¹ for biomass (Tijhuis et al. 1993)
^dCalculated from metabolic network model analysis
^eFrom the reference (Mangiapia and Scott 2016)