AMB Express

Table 4 Olsenella scatoligenes (log cell/g digesta), skatole (mmol/kg digesta), and indole (mmol/kg digesta) concentrations in cecal and colonic digesta of pigs fed the control or Chicory

From: Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet

Diet¹	Control				Chicory
Sex	Female		Male		Female		Male		p value
Weight (kg)	60 (n = 3)	120 (n = 2)	60 (n = 3)	120 (n = 4)	60 (n = 2)	120 (n = 3)	60 (n = 4)	120 (n = 3)	Diet	Sex	Weight	D*S	D*W	S*W	DSW
Caecum
O. scatoligenes	4.84 ± 0.28^a	4.74 ± 0.23^a	5.02 ± 0.20^a	4.73 ± 0.23^a	5.38 ± 0.23^a	4.61 ± 0.28^a	6.77 ± 0.23^b	4.74 ± 0.20^a	0.005	0.023	< 0.001	0.061	0.003	0.047	0.135
Skatole	0.8 ± 0.9	2.4 ± 0.7	2.3 ± 0.6	2.9 ± 0.7	0.0 ± 0.7	0.0 ± 0.9	0.0 ± 0.7	0.0 ± 0.6	0.001	0.360	0.315	0.360	0.315	0.644	0.644
Indole	2.7 ± 1.6	2.9 ± 1.3	5.4 ± 1.6	3.7 ± 1.3	0.7 ± 1.3	1.6 ± 1.6	0.5 ± 1.3	0.4 ± 1.2	0.008	0.584	0.860	0.209	0.573	0.468	0.786
Colon
O. scatoligene	6.01 ± 0.13^a	5.76 ± 0.11^a	5.93 ± 0.09^a	5.83 ± 0.11^a	6.12 ± 0.11^a	5.97 ± 0.13^a	7.23 ± 0.11^b	6.02 ± 0.11^a	< 0.001	0.002	< 0.001	0.002	0.006	0.010	0.002
Skatole	16.5 ± 5.3	29.4 ± 4.3	21.9 ± 3.7	24.7 ± 4.3	0.0 ± 4.3	1.0 ± 5.3	0.2 ± 4.3	0.2 ± 3.7	< 0.001	0.990	0.202	0.917	0.257	0.392	0.473
Indole	6.0 ± 1.9	7.7 ± 1.6	10.1 ± 1.4	5.1 ± 1.6	0.3 ± 1.6	1.2 ± 1.9	0.2 ± 1.6	0.3 ± 1.4	< 0.001	0.888	0.610	0.586	0.361	0.120	0.217

Values are least square means and standard error
¹Experimental diets: control = a standard Danish grower diet based on wheat, barley, and soybean meal; chicory = the same diet in which barley and wheat had been substituted with 25% chicory root
^{a, b}Means within a row without a common superscript letter differ (p < 0.05)

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