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Table 3 DNA recovery rates from serially diluted O. scatoligenes SK9K4T cells spiked into colonic digesta

From: Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet

Spikeda (cells/reaction)

Detectedb (copies/reaction)

Recoveryc (%)

3.0 × 107

(2.64 ± 0.45) × 106

8.8 ± 1.5

3.0 × 106

(2.86 ± 0.59) × 105

9.5 ± 2.0

3.0 × 105

(2.67 ± 0.42) × 104

8.9 ± 1.4

3.0 × 104

(1.92 ± 0.49) × 103

6.4 ± 1.6

  1. aThree 200 mg colonic digesta samples were spiked with 3 × 102–3 × 109 cells of SK9K4T, giving spiked concentrations at 3 × 100 to 3 × 107 cells per PCR reaction. Samples with values below 3.0 × 104 cells per PCR reaction are not shown due to abundance of indigenous O. scatoligenes in colon
  2. bDetected O. scatoligenes genomic copies/reaction was obtained from gDNA standard curve
  3. cRecovery rate was calculated as the quotient of recovered copies and spiked cells
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