Fig. 2From: Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based dietSpecificity of the primer pair alone and TaqMan primerâprobe pair for Olsenella scatoligenes SK9K4T using genomic DNA targeting a specific region of the 16s rRNA gene sequence. a Conventional PCR with the primer pair alone. 1, O. scatoligenes; 2, O. uli; 3, O. profusa; 4â12, non-specific cultures; 13, distilled water. b SYBR Green real-time PCR with the primer pair alone. c TaqMan-MGB real-time PCR with the primerâprobe pairBack to article page