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Fig. 2 | AMB Express

Fig. 2

From: Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet

Fig. 2

Specificity of the primer pair alone and TaqMan primer–probe pair for Olsenella scatoligenes SK9K4T using genomic DNA targeting a specific region of the 16s rRNA gene sequence. a Conventional PCR with the primer pair alone. 1, O. scatoligenes; 2, O. uli; 3, O. profusa; 4–12, non-specific cultures; 13, distilled water. b SYBR Green real-time PCR with the primer pair alone. c TaqMan-MGB real-time PCR with the primer–probe pair

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