Fig. 2From: Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coliConstruction of recombinant plasmids with different gene order of crtE, crtB and crtI. The fragments crtE1, crtE2, crtE3, crtB1, crtB2, crtB3, crtI1, crtI2 and crtI3 with different restriction enzyme sites were amplified and cloned into pET22b to form six recombinant plasmids with different gene orders of crtE, crtB and crtI. The termination codon TAA of the first and second genes was removedBack to article page