Fig. 1
Biotransformation of methyl decanoate to sebacic acid using an evolved Candida tropicalis strain with decane induction method. This experiment was adapted from that described in a previous report after several modifications (Beardslee et al. 2014). After the completion of the growth phase, decane induction was conducted for 10 h using 10% v/v decane. Thereafter, methyl decanoate was fed slowly (and subsequently increased in a sequential manner) after decane induction to avoid the accumulation of decanoic acid. Error bars represent standard deviation of three measurements from two independent runs. OD optical density, SA sebacic acid, DA decanoic acid, 10-HDA 10-hydroxydecanoic acid, MD methyl decanoate. Symbols:
: optical density;
: sebacic acid concentration;
: 10-hydroxydecanoic acid concentration;
: decanoic acid concentration;
: decane concentration;
: methyl decanoate feeding rate; *methyl decanoate feeding rate was counted on the initial volume basis