Table 1 Primers used for construction of overexpression plasmid
Primer symbol | Primers (5′-3′) |
|---|---|
VF1 | GGTGGCACTAGAGAAGACTATTTTTG |
VR1 | GAATTCGGAAGCGGGCAGTG |
hygF | TTCTCTAGTGCCACCATGAAAAAGCCTGAACTCACCG |
hygR | CCCGCTTCCGAATTCTTCCTTTGCCCTCGGAC |
VF2 | GGATCCGGGGCCGGGGTTGCTC |
VR2 | ACGCGTTCCGGAAATCAACCTCTGG |
amy365-1F | CCCGGCCCCGGATCCATGTCGGCTCCTCGTCCACGAGGC |
amy365-1R | ATTTCCGGAACGCGTTTATTGCCATGTACTATTCACACTCGAAGCC |
vgbF | CCCGGCCCCGGATCCATGCTTGATCAACAAACGATCAACA |
vgbR | ATTTCCGGAACGCGTCTATTCGACGGCTTGGGCGT |
TtrpCF | ATCCACTTAACGTTACTGAAATCATC |
TtrpCR | CATTTTTCGACAATCCGAGTGGAGATGTGGGAG |
PgpdAF | GATTGTCGAAAAATGTAAAGG |
PgpdAR | GATGAAGTGTTTGTTGAGCTAGGGGAG |
amy365-1R1 | TAACGTTAAGTGGATTTATTGCCATGTACTATTCACACTCGAAGCC |
vgbF1 | AACAAACACTTCATCATGCTTGATCAACAAACGATCAACA |