Ribosomal binding site sequences and promoters for expressing glutamate decarboxylase and producing γ-aminobutyrate in Corynebacterium glutamicum

AMB Express

Table 3 GABA production in several recombinant C. glutamicum strains

Strains	GAD	GAD ΔpknG	Harbouring pHGmut	R4a-B2	R4a-B2B1^mut	GABA6C	GABA6F
Cultivation in	Shake flask		Fed-batch	Shake flask		Fed-batch
Pathway	GAD		GAD	GAD		Putrescine
Cultivation time (h)	120	120	72	72	60	64	69
GABA titer (g/L)	13.1 ± 0.5	31.2 ± 0.4	38.6 ± 0.9	20.2 ± 0.3	26.5 ± 1.0	59.7	63.2
GABA volumeric productivity (g/L/h)	0.108	0.259	0.536	0.281	0.442	1.34	1.13
GABA yield on glucose (g/g)	0.156	0.511	0.320	0.212	0.269	0.24	0.24
References	Okai et al. (2014)		Choi et al. (2015)	This work		Jorge et al. (2017)