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Table 3 GABA production in several recombinant C. glutamicum strains

From: Ribosomal binding site sequences and promoters for expressing glutamate decarboxylase and producing γ-aminobutyrate in Corynebacterium glutamicum

Strains GAD GAD ΔpknG Harbouring pHGmut R4a-B2 R4a-B2B1mut GABA6C GABA6F
Cultivation in Shake flask Fed-batch Shake flask Fed-batch
Pathway GAD GAD GAD Putrescine
Cultivation time (h) 120 120 72 72 60 64 69
GABA titer (g/L) 13.1 ± 0.5 31.2 ± 0.4 38.6 ± 0.9 20.2 ± 0.3 26.5 ± 1.0 59.7 63.2
GABA volumeric productivity (g/L/h) 0.108 0.259 0.536 0.281 0.442 1.34 1.13
GABA yield on glucose (g/g) 0.156 0.511 0.320 0.212 0.269 0.24 0.24
References Okai et al. (2014) Choi et al. (2015) This work Jorge et al. (2017)