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Table 1 Primers used in this study

From: Salinity effect on the metabolic pathway and microbial function in phenanthrene degradation by a halophilic consortium

Gene targets Primers Sequences Amplicon length Annealing temperatures °C References
RHD pahAc-f 5′-ATTGCGCTTAYCAYGGBTGG-3′ 400 49 Ding et al. (2010)
pahAc-r 5′-ATAGGTGTCTCCAACRAARTT-3′
C12O C12O-f 5′-ACCATCGARGGYCCSCTSTAY-3′ 500 60 Sei et al. (1999)
C12O-r 5′-GTTRATCTGGGTGGTSAG-3′
C23O C23O-f 5′-CGACCTGATCRSCATGACCGA-3′ 280 50 García et al. (2005)
C23O-r 5′-TYAGGTCAKMACGGTCA-3′
G12O G12O-f 5′-CGRTGATSTGGMTSGAYGG-3′ 460 53 This study
G12O-r 5′-CCAGCYVGGMACCACRAASA -3′
P34O P34O-f 5′-GCSCCSCTSGAGCCSAACTTC-3′ 500 60 Azhari et al. (2007)
P34O-r 5′-GCCGCGSAGSACGATRTCGAA-3′
q-C12O Q-C12f 5′-CCAGAGCGAATACAACATG-3′ 174 50 This study
Q-C12r 5′-GGAGACGAAGTAGTGGATG-3′
q-C23O Q-C23f 5′-TGTGGTGGTGACTACTTCT-3′ 104 57 This study
Q-C23r 5′-AAGCGTTCGTTCAGTACC-3′
q-G12O Q-Gf 5′-GAACAATCGCCAGGAAGT-3′ 237 51 This study
Q-Gr 5′-CATGGTAAGGATCGGTGAT-3′
q-P34O Q-Pf 5′-AGCACGGCTACTACCATT-3′ 191 57 This study
Q-Pr 5′-CGATACGCAGTTCACGAT-3′