Table 1 Primers used in this study
Gene targets | Primers | Sequences | Amplicon length | Annealing temperatures °C | References |
|---|---|---|---|---|---|
RHD | pahAc-f | 5′-ATTGCGCTTAYCAYGGBTGG-3′ | 400 | 49 | Ding et al. (2010) |
pahAc-r | 5′-ATAGGTGTCTCCAACRAARTT-3′ | ||||
C12O | C12O-f | 5′-ACCATCGARGGYCCSCTSTAY-3′ | 500 | 60 | Sei et al. (1999) |
C12O-r | 5′-GTTRATCTGGGTGGTSAG-3′ | ||||
C23O | C23O-f | 5′-CGACCTGATCRSCATGACCGA-3′ | 280 | 50 | GarcÃa et al. (2005) |
C23O-r | 5′-TYAGGTCAKMACGGTCA-3′ | ||||
G12O | G12O-f | 5′-CGRTGATSTGGMTSGAYGG-3′ | 460 | 53 | This study |
G12O-r | 5′-CCAGCYVGGMACCACRAASA -3′ | ||||
P34O | P34O-f | 5′-GCSCCSCTSGAGCCSAACTTC-3′ | 500 | 60 | Azhari et al. (2007) |
P34O-r | 5′-GCCGCGSAGSACGATRTCGAA-3′ | ||||
q-C12O | Q-C12f | 5′-CCAGAGCGAATACAACATG-3′ | 174 | 50 | This study |
Q-C12r | 5′-GGAGACGAAGTAGTGGATG-3′ | ||||
q-C23O | Q-C23f | 5′-TGTGGTGGTGACTACTTCT-3′ | 104 | 57 | This study |
Q-C23r | 5′-AAGCGTTCGTTCAGTACC-3′ | ||||
q-G12O | Q-Gf | 5′-GAACAATCGCCAGGAAGT-3′ | 237 | 51 | This study |
Q-Gr | 5′-CATGGTAAGGATCGGTGAT-3′ | ||||
q-P34O | Q-Pf | 5′-AGCACGGCTACTACCATT-3′ | 191 | 57 | This study |
Q-Pr | 5′-CGATACGCAGTTCACGAT-3′ |