Fig. 1From: Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedensa SDS-PAGE and b Western blot analyses of rAgaB-4 expression in E. coli BL21(DE3). Lane M PageRuler™ Prestained Protein Ladder; lanes 1 and 2 total cell lysates of E. coli BL21(DE3) containing pET-29a(+) induced by IPTG at 37 °C for 4 and 24 h, respectively; lanes 3 and 4 total cell lysates of E. coli BL21(DE3) containing pET-AGAB-4 induced by IPTG at 37 °C for 4 and 24 h, respectively. Arrows indicate rAgaB-4 expressionBack to article page