Fig. 4
From: Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
Anti-Mycobacterium bovis capacity of rHBD3 protein to A549 cells. a M. bovis growth curve after incubation with different concentrations of HBD-3. Negative control is M. bovis with Middle Brook 7H9 broth. Positive control is M. bovis with streptomycin (1000 U/ml). b Mycobacteria invasion tests. Yellow spots in the figure are Mycobacteria. c Cell apoptosis analysis of A549 cells infected by M. bovis with different treatments. d The data of cell apoptosis and death ratio analysis of A549 cells infected by M. bovis with different treatments. All the experiments were replicated three times and the changes are presented as mean ± SEM. P values < 0.05 were considered as statistically significant. e CFU tests in A549 cells and its medium (*P < 0.05)