Fig. 1From: Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacityRecombinant HBD3 protein prokaryotic expression vector construction and verification. a Designing scheme of rHBD3 prokaryotic expression vector. b Restriction enzyme analysis of the PGEX-5X-HBD3 recombinant plasmid. Lane 1 is the DNA fragment recombination plasmid digested by EcoRI. Lane 2 is the DNA fragments of recombination plasmid digested by BstBI and EcoRI. c Sequencing identification of PGEX-5X-HBD3 vector. The DNA and amino acid fragments marked by red are the Factor Xa recognition sites. The yellow one is the HBD3 protein sequencesBack to article page