Table 1 Plasmids and strains used in this study
From: Balanced trafficking between the ER and the Golgi apparatus increases protein secretion in yeast
Name | Description | References |
|---|---|---|
Plasmids | ||
CPOTud | TPI promoter and terminator from S. cerevisiae, POT marker from Schizosaccharomyces pombe (2 μ) | Liu et al. (2012) |
pAlphaAmyCPOT | α factor leader with α-amylase gene inserted into CPOTud | Liu et al. (2012) |
pAlphaTrEGCPOT | α factor leader with endoglucanase I gene inserted into CPOTud | Bao et al. (2017) |
pCP-aGLA | α factor leader with glucan 1,4-α-glucosidase gene inserted into CPOTud | Huang et al. (2015) |
Strains | ||
CEN.PK530-6CK | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP P GPD -SEC16 | Bao et al. (2017) |
YIGS16 | CEN.PK530-6CK with pAlphaAmyCPOT | Bao et al. (2017) |
YIGCS1 | YIGS16 amdSYM P TEF -GCS1 | This study |
YIGLO3 | YIGS16 amdSYM P TEF -GLO3 | This study |
AACK | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP with pAlphaAmyCPOT | Bao et al. (2017) |
AACK-GCS1 | AACK amdSYM P TEF -GCS1 | This study |
AACK-GLO3 | AACK amdSYM P TEF -GLO3 | This study |
YIGLO3GCS1 | YIGS16 P TEF -GLO3 amdSYM P TEF -GCS1 | This study |
CEN.PK530-6CK + EG | CEN.PK530-6CK with pAlphaTrEGCPOT | This study |
CEN.PK530-6CK + AGL | CEN.PK530-6CK with pCP-aGLA | This study |
GLO3 + EG | CEN.PK530-6CK amdSYM P TEF -GLO3 with pAlphaTrEGCPOT | This study |
GLO3 + AGL | CEN.PK530-6CK amdSYM P TEF -GLO3 with pCP-aGLA | This study |