Biochemical and mutational analyses of a Trametes pyranose oxidase and comparison of its mutants in breadmaking

AMB Express

Table 2 Effect of metal ions and chemical reagents on the activity of TsPOx

Chemicals	Relative activity (%)^a		Chemicals	Relative activity (%)
Chemicals	5 mM	10 mM	Chemicals	5 mM	10 mM
Control	100	100	Cu²⁺	104.9 ± 0.6	101.7 ± 0.8
Ni²⁺	119.5 ± 2.3	125.4 ± 1.7	Zn²⁺	103.5 ± 1.2	123.2 ± 1.6
Mg²⁺	113.0 ± 2.3	120.6 ± 1.6	Cr³⁺	100.0 ± 0.6	61.6 ± 0.8
Mn²⁺	113.0 ± 1.3	123.3 ± 1.1	Ag⁺	94.9 ± 1.2	51.4 ± 1.3
K⁺	110.3 ± 2.2	127.6 ± 1.3	Al³⁺	93.0 ± 0.6	73.0 ± 1.1
Na⁺	108.1 ± 2.3	125.4 ± 1.7	Fe²⁺	11.6 ± 0.3	8.4 ± 0.3
Ca²⁺	106.5 ± 0.6	120.0 ± 0.8	EDTA	85.3 ± 0.7	63.8 ± 2.4
Co²⁺	106.5 ± 2.3	122.2 ± 0.6	SDS	0	0