|
Run
|
Models
|
Metal ions concentration (mM)
|
Fluorescence intensity (AU)
|
|---|
|
Mn2+ (x1)
|
Fe2+ (x2)
|
Ca2+ (x3)
|
Mg2+ (x4)
|
Experimental
|
Predicted
|
|---|
|
1
|
0000
|
1
|
3
|
2
|
3
|
299.2 ± 44.7
|
299.1
|
|
2
|
0a00
|
1
|
1.52
|
2
|
3
|
292.1 ± 38.3
|
290.3
|
|
3
|
−−++
|
0.5
|
2
|
3
|
4
|
280.8 ± 21.2
|
278.4
|
|
4
|
+−++
|
1.5
|
2
|
3
|
4
|
284.4 ± 38.2
|
286.3
|
|
5
|
++++
|
1.5
|
4
|
3
|
4
|
282.3 ± 30.0
|
282.2
|
|
6
|
−+++
|
0.5
|
4
|
3
|
4
|
268.2 ± 24.3
|
268.0
|
|
7
|
−−−+
|
0.5
|
2
|
1
|
4
|
263.4 ± 20.2
|
265.1
|
|
8
|
++−−
|
1.5
|
4
|
1
|
2
|
280.8 ± 28.6
|
282.0
|
|
9
|
++−+
|
1.5
|
4
|
1
|
4
|
281.5 ± 30.4
|
276.7
|
|
10
|
000A
|
1
|
3
|
2
|
4.48
|
288.5 ± 27.6
|
290.4
|
|
11
|
−−+−
|
0.5
|
2
|
3
|
2
|
280.5 ± 23.3
|
284.4
|
|
12
|
a000
|
0.26
|
3
|
2
|
3
|
278.8 ± 20.1
|
278.8
|
|
13
|
+−−+
|
1.5
|
2
|
1
|
4
|
266.4 ± 17.4
|
265.8
|
|
14
|
0000
|
1
|
3
|
2
|
3
|
302.9 ± 33.1
|
299.1
|
|
15
|
−+−−
|
0.5
|
4
|
1
|
2
|
287.2 ± 25.7
|
284.4
|
|
16
|
+−+−
|
1.5
|
2
|
3
|
2
|
284.5 ± 24.0
|
283.1
|
|
17
|
+−−−
|
1.5
|
2
|
1
|
2
|
271.0 ± 18.4
|
270.4
|
|
18
|
+++−
|
1.5
|
4
|
3
|
2
|
282.2 ± 24.6
|
279.6
|
|
19
|
00a0
|
1
|
3
|
0.52
|
3
|
273.9 ± 23.6
|
277.6
|
|
20
|
0A00
|
1
|
4.48
|
2
|
3
|
285.5 ± 23.3
|
291.2
|
|
21
|
A000
|
1.74
|
3
|
2
|
3
|
279.1 ± 26.0
|
282.9
|
|
22
|
−−−−
|
0.5
|
2
|
1
|
2
|
280.1 ± 4.7
|
279.0
|
|
23
|
−++−
|
0.5
|
4
|
3
|
2
|
275.2 ± 17.4
|
274.6
|
|
24
|
00A0
|
1
|
3
|
3.48
|
3
|
285.4 ± 19.5
|
285.6
|
|
25
|
−+−+
|
0.5
|
4
|
1
|
4
|
269.6 ± 15.3
|
269.8
|
|
26
|
000a
|
1
|
3
|
2
|
1.52
|
296.9 ± 24.5
|
298.8
|
|
27
|
0000
|
1
|
3
|
2
|
3
|
302.2 ± 26.6
|
299.1
|
- The experimental results are the means of two replicates of 1000-fold diluted spore samples. The symbols in the model column mean each factor at five different levels (−a, −, 0, +, A). The variables at a central coded value are considered at zero
