Table 1 Technical details for microbial community characterization
Primers | Target taxa | PCR conditions | References |
|---|---|---|---|
Arch-amoAF Arch-amoAR | AOA | 94 °C for 2 min; followed by 30–35 cycles for 30 s at 94 °C, 45 s at 53 °C, and 45 s at 72 °C, followed by 10 min of final extension at 72 °C | Francis et al. (2005) |
amoA-1F amoA-2R | AOB | 94 °C for 2 min; followed by 30–35 cycles of 30 s at 94 °C, 45 s at 55 °C, and 45 s at 72 °C, followed by 10 min of final extension at 72 °C | Rotthauwe et al. (1997) |