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Table 1 Technical details for microbial community characterization

From: Ammonia-oxidizing bacteria and archaea within biofilters of a commercial recirculating marine aquaculture system

Primers Target taxa PCR conditions References
Arch-amoAF
Arch-amoAR
AOA 94 °C for 2 min; followed by 30–35 cycles for 30 s at 94 °C, 45 s at 53 °C, and 45 s at 72 °C, followed by 10 min of final extension at 72 °C Francis et al. (2005)
amoA-1F
amoA-2R
AOB 94 °C for 2 min; followed by 30–35 cycles of 30 s at 94 °C, 45 s at 55 °C, and 45 s at 72 °C, followed by 10 min of final extension at 72 °C Rotthauwe et al. (1997)
  1. AOA ammonia-oxidizing archaea, AOB ammonia-oxidizing bacteria