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Table 2 Results of the toxicity test (test organism: Vibrio fischeri), including concentration of DCF and BPA, before and after laccase treatment in a membrane reactor (volume: 10 l; phosphate buffer; incubation time: 4 h)

From: Enhanced laccase-mediated transformation of diclofenac and flufenamic acid in the presence of bisphenol A and testing of an enzymatic membrane reactor

Reaction

Concentration

Toxicity test (Vibrio fischeri)

DCF

BPA

EC50

EC20

mg/l

mM

mg/l

mM

mg/l

mM

mg/l

mM

DCF

 Before laccase treatment

31.81

0.10

  

11.36

0.0357

5.53

0.0174

 After laccase treatment

21.70

0.07

  

19.50

0.0613

9.59

0.0302

BPA

 Before laccase treatment

  

22.83

0.10

5.24

0.0230

1.36

0.0060

 After laccase treatment

  

0

0

22.16

0.0971

3.01

0.0132

DCF and BPA

 Before laccase treatment

31.81

0.10

22.83

0.10

3.91

0.0143

1.50

0.0055

 After laccase treatment

15.27

0.05

0

0

12.41

0.0454

6.24

0.0225