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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Conformational determinants necessary for secretion of Paecilomyces thermophila β-1,4-xylosidase that lacks a signal peptide

Fig. 3

Subcellular locations of the X12345–ChiX, GFP, AIO6, hIL-2. Each of the for ‘passenger’ proteins, AIO6, hIL-2, and GFP, were expressed in E. coli BL21(DE3) harboring pET28a(+) or pX12345LT. The lane loadings are directly comparable. SF soluble fraction, IF insoluble fraction, C cells, M medium, P1 periplasm fraction (hypertonic solution fraction), P2 periplasm fraction (hypotonic solution fraction), S spheroplasts

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