Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

Yang, En; Fan, Lihua; Yan, Jinping; Jiang, Yueming; Doucette, Craig; Fillmore, Sherry; Walker, Bradley

doi:10.1186/s13568-018-0536-0

AMB Express

Table 1 Growth kinetics of the four bacteriocinogenic LAB strains under different growth conditions

From: Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria

Isolates	Arla-10			JFR-1			JFR-5			TSB-8
Culture conditions	Slope^a	Latency time (h)	A ^b_max	Slope	Latency time (h)	A_max	Slope	Latency time (h)	A_max	Slope	Latency time (h)	A_max
20 °C, MRS pH 5.5	1.37	25.30	0.63	1.17	25.01	0.58	0.87	22.21	0.79	0.05	5.41	0.16
20 °C, MRS pH 6.2	0.97	11.66	1.04	0.77	11.74	0.89	0.77	16.41	0.88	0.01	4.31	0.17
20 °C, MRS pH 7.4	1.05	10.28	1.22	1.08	11.29	1.22	0.59	25.27	0.71	0.06	4.00	0.19
20 °C, MRS pH 8.5	0.96	11.66	1.15	0.91	12.01	1.12	0.56	21.76	0.68	0.01	5.97	0.24
20 °C, BHI pH 5.5	0.54	8.33	0.62	0.58	8.61	0.66	0.44	14.10	0.46	0.09	19.72	0.16
20 °C, BHI pH 6.2	0.80	6.53	0.94	0.97	5.79	0.99	0.59	13.02	0.62	0.13	21.88	0.16
20 °C, BHI pH 7.4	0.82	8.01	0.92	0.84	7.91	0.93	0.54	15.09	0.55	0.51	38.52	0.23
20 °C, BHI pH 8.5	0.78	8.94	0.88	0.80	9.08	0.90	0.42	14.78	0.47	0.62	43.25	0.24
37 °C, MRS pH 5.5	0.56	3.85	0.67	0.56	3.81	0.67	0.42	7.83	0.45	0.32	2.96	0.43
37 °C, MRS pH 6.2	0.92	3.07	1.03	0.92	3.11	1.03	0.68	7.39	0.79	0.44	5.50	0.58
37 °C, MRS pH 7.4	0.95	3.00	1.10	0.91	3.01	1.06	0.83	7.50	1.00	0.67	4.30	0.65
37 °C, MRS pH 8.5	0.92	3.74	1.15	0.93	4.10	1.15	0.77	8.56	1.00	0.47	5.63	0.68
37 °C, BHI pH 5.5	0.70	2.73	0.79	0.75	2.47	0.82	0.62	5.39	0.71	0.44	5.69	0.48
37 °C, BHI pH 6.2	0.93	2.39	1.02	0.92	2.35	1.01	0.81	4.70	0.93	0.62	6.30	0.70
37 °C, BHI pH 7.4	0.99	2.63	1.09	1.00	2.66	1.09	0.94	5.19	1.05	0.67	9.57	0.68
37 °C, BHI pH 8.5	0.95	2.85	1.06	0.94	2.85	1.04	0.89	5.55	0.99	0.72	4.16	0.82
44 °C, MRS pH 5.5	0.05	2.37	0.16	0.27	2.31	0.39	0.10	2.70	0.24	0.40	3.96	0.41
44 °C, MRS pH 6.2	0.31	2.53	0.45	0.52	2.13	0.66	0.23	3.07	0.38	0.50	3.15	0.36
44 °C, MRS pH 7.4	0.55	2.66	0.70	0.75	2.38	0.92	0.30	3.63	0.47	0.39	2.45	0.48
44 °C, MRS pH 8.5	0.58	3.51	0.79	0.75	3.18	0.97	0.25	6.06	0.47	0.51	2.90	0.23
44 °C, BHI pH 5.5	0.64	2.38	0.78	0.80	2.18	0.89	0.45	1.46	0.55	0.65	2.21	0.59
44 °C, BHI pH 6.2	0.62	2.33	0.71	0.85	1.90	0.93	0.43	1.47	0.51	0.48	2.33	0.56
44 °C, BHI pH 7.4	0.59	2.32	0.67	0.82	2.11	0.90	0.36	1.40	0.44	0.46	2.68	0.46
44 °C, BHI pH 8.5	0.66	2.37	0.75	0.86	2.33	0.94	0.39	1.13	0.48	0.42	4.32	0.50

^a The slope of growth curves
^b OD (the maximum absorbency under λ_{600 nm})

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