Lysis buffer/agent
|
STES buffer
|
TN150 buffer
|
Tissue lysis buffer and protinase K
|
Distilled water
|
Aqueous NaOH
|
TNES buffer and protinase K
|
Cell lysis and homogenization
|
Bead beating
|
Bead beating
|
Incubation at 56 °C and vortexing
|
Boiling
|
Boiling
|
Incubation at 56 °C and vortexing
|
Extraction and DNA precipitation
|
Phenol chloroform and cold absolute ethanol
|
Phenol chloroform and cold absolute ethanol
|
Mini column and washing buffer
|
Heat
|
Heat
|
Hypertonic NaCl and cold absolute ethanol
|
Store in
|
TE buffer
|
TE buffer
|
Elution buffer
|
Distilled water
|
Aqueous NaOH
|
TE buffer
|
Approximate time for completion
|
7 ½ h
|
8 h
|
3 h
|
25 min
|
35 min
|
11 h
|