Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method

AMB Express

Table 1 Comparison of six different DNA extraction methods examined in this study

Extraction steps	BP₁	BP₂	K	H₁	H₂	S
Lysis buffer/agent	STES buffer	TN150 buffer	Tissue lysis buffer and protinase K	Distilled water	Aqueous NaOH	TNES buffer and protinase K
Cell lysis and homogenization	Bead beating	Bead beating	Incubation at 56 °C and vortexing	Boiling	Boiling	Incubation at 56 °C and vortexing
Extraction and DNA precipitation	Phenol chloroform and cold absolute ethanol	Phenol chloroform and cold absolute ethanol	Mini column and washing buffer	Heat	Heat	Hypertonic NaCl and cold absolute ethanol
Store in	TE buffer	TE buffer	Elution buffer	Distilled water	Aqueous NaOH	TE buffer
Approximate time for completion	7 ½ h	8 h	3 h	25 min	35 min	11 h

BP ₁ Bead beater phenol chloroform with STES buffer, BP ₂ Bead beater phenol chloroform with TN150 buffer, K DNeasy blood and tissue kit, H ₁ Heat treating in distilled water, H ₂ Heat treating in NaOH, S Salting out method