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Table 1 Comparison of six different DNA extraction methods examined in this study

From: Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method

Extraction steps BP1 BP2 K H1 H2 S
Lysis buffer/agent STES buffer TN150 buffer Tissue lysis buffer and protinase K Distilled water Aqueous NaOH TNES buffer and protinase K
Cell lysis and homogenization Bead beating Bead beating Incubation at 56 °C and vortexing Boiling Boiling Incubation at 56 °C and vortexing
Extraction and DNA precipitation Phenol chloroform and cold absolute ethanol Phenol chloroform and cold absolute ethanol Mini column and washing buffer Heat Heat Hypertonic NaCl and cold absolute ethanol
Store in TE buffer TE buffer Elution buffer Distilled water Aqueous NaOH TE buffer
Approximate time for completion 7 ½ h 8 h 3 h 25 min 35 min 11 h
  1. BP 1 Bead beater phenol chloroform with STES buffer, BP 2 Bead beater phenol chloroform with TN150 buffer, K DNeasy blood and tissue kit, H 1 Heat treating in distilled water, H 2 Heat treating in NaOH, S Salting out method