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Fig. 3 | AMB Express

Fig. 3

From: Improving extracellular production of Serratia marcescens lytic polysaccharide monooxygenase CBP21 and Aeromonas veronii B565 chitinase Chi92 in Escherichia coli and their synergism

Fig. 3

Alignment of CBP21 from S. marcescens (PDB ID: 2BEM), GbpA from Vibrio cholerae (PDB ID: 2XWX) (Wong et al. 2012), Efcbm33a from Enterococcus faecalis (PDB ID: 4A02) (Vaaje-Kolstad et al. 2012), Chitin Binding Domain from Burkholderia pseudomallei (PDB ID: 3UAM) and CBP from A. veronii B565 using PROMALS3D (http://prodata.swmed.edu/promals3d/promals3d.php). Predicted secondary structures: red h: alpha-helix; blue e: beta-strand. Residues involved in chitin binding based on a 2H/1H exchange experiment (Q53, Q57, S58, L110, T111, A112, H114 and T116 of CBP21) (Aachmann et al. 2012) or determined by site-directed mutagenesis (Y54, E55, E60, H114, D182 and N185 of CBP21) (Vaaje-Kolstad et al. b) or highly conserved residues on the CBP21 substrate binding surface determined by ConSurf analysis (H28, E55, S58, E60, T111, A112, H114, D182, T183, N185 and F186 of CBP21) (Ashkenazy et al. 2010) showed by yellow surface coloring

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