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Table 2 List of primers used in this study

From: Application of the thermostable β-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions

Name Sequence 5′–3′ Target
Primers
 Beta-gal_fwd_USER AGCTAUGAACGTTTTATCCTCAATTTGTTACGG bgaB, with flanks for insertion into pUC19
 Beta-gal_rev_USER ACTACTCUAAACCTTCCCGGCTTCATC
 pUC19_fwd_USER ATAGCUGTTTCCTGTGTGAAATTGTTATCCG pUC19
 pUC19_fwd_USER AGAGTAGUTAAGCCAGCCCCGAC
 PNJ24b AATTCGUAATCATGGTCATAGCTGTTTCC pUCG18 backbone with terminator
 PNJ27c AGGGCTTUTGAGCCTTTCATTGAGGCTGTC
 PNJ23 ACCCGGGGAUCCTCTAG pMTL backbone
 PNJ24d AATTCGUAATCATGGTCATATGGATACAGCG
 PNJ27b AGGCTTUTGAGCCTTTCATTGAGG Terminator of groEL gene forward
 PNJ567 AGGAGGUCGTTTCCCATGAACGTTTTATCCTCAATTTGTTACGG bgaB, with flanks for insertion into pUCG18
 PNJ311 AAAGCCCUAAACCTTCCCGGCTTCATCATGCTCTC
 PNJ267 ACGAATUCGGCAAAACAACCGGCTCCTTTTGCTC PgroES with CIRCE deleted
 PNJ268 ACGATAGUTTTCGCCGTTCTTACACACTTATAATATTAATGAACTTCTTTCCGTTTTGC
 PNJ269 ACTATCGUTAAGGAGGTCGTTTCCCATGAGTAAAGGCGAAGAGCTGTTCAC
 PNJ388 ACACACUWWWWATATTAWWN15TTGCAANWWNNWWWTGCAAAAAAATAACTGTTTTTCTCTCCTAAAGAAGAAAG PgroES with randomized sequences
 PNJ389 AGTGTGUAAGAACGGCGAAAACTATCGTTAAG
 PNJ383 AGAGGCUACTCTCAAAAGGTCGGTTTAGACG Terminator of groEL gene reverse
 PNJ566 ACCTCCUTAACGATAGTTTTCGCC PgroES reverse
 PNJ672 AGGAGGUCGTTTCCCATGACCATGATTACGGATTCACTGG lacZ
 PNJ673 AAAGCCUTATTTTTGACACCAGACCAACTGG