Application of the thermostable β-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions

AMB Express

Table 2 List of primers used in this study

Name	Sequence 5′–3′	Target
Primers
Beta-gal_fwd_USER	AGCTAUGAACGTTTTATCCTCAATTTGTTACGG	bgaB, with flanks for insertion into pUC19
Beta-gal_rev_USER	ACTACTCUAAACCTTCCCGGCTTCATC	bgaB, with flanks for insertion into pUC19
pUC19_fwd_USER	ATAGCUGTTTCCTGTGTGAAATTGTTATCCG	pUC19
pUC19_fwd_USER	AGAGTAGUTAAGCCAGCCCCGAC	pUC19
PNJ24b	AATTCGUAATCATGGTCATAGCTGTTTCC	pUCG18 backbone with terminator
PNJ27c	AGGGCTTUTGAGCCTTTCATTGAGGCTGTC	pUCG18 backbone with terminator
PNJ23	ACCCGGGGAUCCTCTAG	pMTL backbone
PNJ24d	AATTCGUAATCATGGTCATATGGATACAGCG	pMTL backbone
PNJ27b	AGGCTTUTGAGCCTTTCATTGAGG	Terminator of groEL gene forward
PNJ567	AGGAGGUCGTTTCCCATGAACGTTTTATCCTCAATTTGTTACGG	bgaB, with flanks for insertion into pUCG18
PNJ311	AAAGCCCUAAACCTTCCCGGCTTCATCATGCTCTC	bgaB, with flanks for insertion into pUCG18
PNJ267	ACGAATUCGGCAAAACAACCGGCTCCTTTTGCTC	P_groES with CIRCE deleted
PNJ268	ACGATAGUTTTCGCCGTTCTTACACACTTATAATATTAATGAACTTCTTTCCGTTTTGC
PNJ269	ACTATCGUTAAGGAGGTCGTTTCCCATGAGTAAAGGCGAAGAGCTGTTCAC
PNJ388	ACACACUWWWWATATTAWWN₁₅TTGCAANWWNNWWWTGCAAAAAAATAACTGTTTTTCTCTCCTAAAGAAGAAAG	P_groES with randomized sequences
PNJ389	AGTGTGUAAGAACGGCGAAAACTATCGTTAAG	P_groES with randomized sequences
PNJ383	AGAGGCUACTCTCAAAAGGTCGGTTTAGACG	Terminator of groEL gene reverse
PNJ566	ACCTCCUTAACGATAGTTTTCGCC	P_groES reverse
PNJ672	AGGAGGUCGTTTCCCATGACCATGATTACGGATTCACTGG	lacZ
PNJ673	AAAGCCUTATTTTTGACACCAGACCAACTGG	lacZ