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Strains
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E. coli TOP10
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F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG
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Thermo Fisher Scientific, USA
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E. coli TOP10: pUC19-bgaB
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E. coli harboring the plasmid pUC19-bgaB
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This study
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G. stearothermophilus DSM2027
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Source of bgaB gene
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DSMZ GmbH, Germany
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G. thermoglucosidans C56-YS93
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Wild type isolate
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Bacillus Genetic Stock Center, Ohio State University, USA
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G. thermoglucosidans: pUCG18P5bgaB
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Strain harboring pUCG18 with P5::bgaB
|
This study
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Plasmids
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pUC19
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General E. coli cloning vector; contains ampicillin resistance gene
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Yanisch-perron et al. (1985)
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pUC19bgaB
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pUC19ΔlacZα plasmid expressing the bgaB gene under control of the pLac promoter.
|
This study
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pUCG18
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E. coli/Geobacillus shuttle vector; AmpR, KanR. Used to construct a library of promoters in E. coli
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Taylor et al. (2008)
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pUCG18P5bgaB
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Template for promoter library
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This study
|
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pMTL61110
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E. coli/Geobacillus shuttle vector; AmpR, KanR. Used for expression in G. thermoglucosidans
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Sheng et al. (2017)
|
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pMTLP13B
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pMTL61110 with P13-bgaB
|
This study
|
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pMTLP24B
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pMTL61110 with P24-bgaB
|
This study
|
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pMTLP27B
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pMTL61110 with P27-bgaB
|
This study
|
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pMTLP13Z
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pMTL61110 with P13-lacZ
|
This study
|
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pMTLP24Z
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pMTL61110 with P24-lacZ
|
This study
|
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pMTLP27Z
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pMTL61110 with P27-lacZ
|
This study
|
