Table 1 List of strains and plasmids
Name | Relevant characteristics | Reference |
|---|---|---|
Strains | ||
E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG | Thermo Fisher Scientific, USA |
E. coli TOP10: pUC19-bgaB | E. coli harboring the plasmid pUC19-bgaB | This study |
G. stearothermophilus DSM2027 | Source of bgaB gene | DSMZ GmbH, Germany |
G. thermoglucosidans C56-YS93 | Wild type isolate | Bacillus Genetic Stock Center, Ohio State University, USA |
G. thermoglucosidans: pUCG18P5bgaB | Strain harboring pUCG18 with P5::bgaB | This study |
Plasmids | ||
pUC19 | General E. coli cloning vector; contains ampicillin resistance gene | Yanisch-perron et al. (1985) |
pUC19bgaB | pUC19ΔlacZα plasmid expressing the bgaB gene under control of the pLac promoter. | This study |
pUCG18 | E. coli/Geobacillus shuttle vector; AmpR, KanR. Used to construct a library of promoters in E. coli | Taylor et al. (2008) |
pUCG18P5bgaB | Template for promoter library | This study |
pMTL61110 | E. coli/Geobacillus shuttle vector; AmpR, KanR. Used for expression in G. thermoglucosidans | Sheng et al. (2017) |
pMTLP13B | pMTL61110 with P13-bgaB | This study |
pMTLP24B | pMTL61110 with P24-bgaB | This study |
pMTLP27B | pMTL61110 with P27-bgaB | This study |
pMTLP13Z | pMTL61110 with P13-lacZ | This study |
pMTLP24Z | pMTL61110 with P24-lacZ | This study |
pMTLP27Z | pMTL61110 with P27-lacZ | This study |