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Table 1 List of strains and plasmids

From: Application of the thermostable β-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions

Name Relevant characteristics Reference
Strains
 E. coli TOP10 F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG Thermo Fisher Scientific, USA
 E. coli TOP10: pUC19-bgaB E. coli harboring the plasmid pUC19-bgaB This study
 G. stearothermophilus DSM2027 Source of bgaB gene DSMZ GmbH, Germany
 G. thermoglucosidans C56-YS93 Wild type isolate Bacillus Genetic Stock Center, Ohio State University, USA
 G. thermoglucosidans: pUCG18P5bgaB Strain harboring pUCG18 with P5::bgaB This study
Plasmids
 pUC19 General E. coli cloning vector; contains ampicillin resistance gene Yanisch-perron et al. (1985)
 pUC19bgaB pUC19ΔlacZα plasmid expressing the bgaB gene under control of the pLac promoter. This study
 pUCG18 E. coli/Geobacillus shuttle vector; AmpR, KanR. Used to construct a library of promoters in E. coli Taylor et al. (2008)
 pUCG18P5bgaB Template for promoter library This study
 pMTL61110 E. coli/Geobacillus shuttle vector; AmpR, KanR. Used for expression in G. thermoglucosidans Sheng et al. (2017)
 pMTLP13B pMTL61110 with P13-bgaB This study
 pMTLP24B pMTL61110 with P24-bgaB This study
 pMTLP27B pMTL61110 with P27-bgaB This study
 pMTLP13Z pMTL61110 with P13-lacZ This study
 pMTLP24Z pMTL61110 with P24-lacZ This study
 pMTLP27Z pMTL61110 with P27-lacZ This study