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Table 1 Kinetic parameters of A. aerogenes sulfatase and detection limits

From: Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays

  Km (μM) Vmax (μM/min) LOD (FI) (ng/ml) LOD (UV) (μg/ml)
Probe 1 187 ± 13 10.9 ± 0.2 35.3 ± 2.6b 1.99 ± 0.09c
MFSa 598 ± 67 (75 ± 4.8) × 10−6 158d  
RSa 162 ± 10 (606 ± 12) × 10−6 15.8d  
p-NPSa 1800 ± 120    0.0158d
  1. aThe values were reported in reference, Smith et al. (2014)
  2. bThe values were calculated by fluorescence intensity changes at 415 nm after 30 min incubation
  3. cThe values were calculated by UV absorption changes at 630 nm after 2 h incubation
  4. dThe values were obtained after 10 min incubation