Table 1 Strains and plasmids used in this study
From: Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum
Strains | Relevant characteristics | Source/reference/application |
|---|---|---|
E. coli | ||
DH5α | Cloning host | Hanahan (1985) |
C. glutamicum | ||
WT | ATCC 13032, wild type | ATCC |
∆sigB | Deletion in sigB | Larisch et al. (2007) |
∆sigE | Deletion in sigE | Park et al. (2008) |
∆sigH | Deletion in sigH | Zemanová et al. (2008) |
∆sigM | Deletion in sigM | Nakunst et al. (2007) |
Plasmids | ||
pEC-XT99A | E. coli–C. glutamicum expression vector, TcR, IPTG-inducible trc promoter | Kirchner and Tauch (2003) |
pEKEx3 | E. coli–C. glutamicum expression vector, SpR, IPTG-inducible tac promoter | Hoffelder et al. (2010) |
pEPR1 | E. coli–C. glutamicum promoter-test vector, KmR, promoterless gfpuv as a reporter | Knoppová et al. (2007) |
pEPR-P2sigA | pEPR1 with P2sigA | This work |
pEPR-Pfba | pEPR1 with Pfba | This work |
pEPR-Pcg2556 | pEPR1 with Pcg2556 | This work |
pEPR-Pcmt1 | pEPR1 with Pcmt1 | This work |
pEPR-PsigB | pEPR1 with PsigB | This work |
pEPR-PrshA | pEPR1 with PrshA | This work |
pEPR-PtrxB1 | pEPR1 with PtrxB1 | This work |
pRLG770 | E. coli vector for in vitro transcription, rrnB terminator, ApR | Ross et al. (1990) |
pRLG770P2sigA | pRLG770 with P2sigA | This work |
pRLG770Pfba | pRLG770 with Pfba | This work |
pRLG770Pcg2556 | pRLG770 with Pcg2556 | This work |
pRLG770Pcmt1 | pRLG770 with Pcmt1 | This work |
pRLG770 PsigB | pRLG770 with PsigB | This work |
pRLG770PrshA | pRLG770 with PrshA | This work |
pRLG770PtrxB1 | pRLG770 with PtrxB1 | This work |