Fig. 7
From: Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum
Assignment of sigma factors to the Pfba promoter. a Sequence of the Pfba promoter (Ehira et al. 2008). b In vitro transcription with Pfba as a template. Individual sigma factors associated with RNAP are shown at the top. The specific transcripts are indicated with an arrow. c Pfba promoter activity determined with the two-plasmid system in C. glutamicum WT (with the sig-constructs in the expression vector pEKEx3) by measuring the fluorescence intensity of the Gfpuv reporter. Ft − F0 is the difference between the fluorescence at the sampling time (3, 6, 24 h) and the fluorescence at time 0 (before sigma gene induction). The sigma factors whose genes were overexpressed are indicated at the bottom. IPTG was added at time 0. d Activity of Pfba in C. glutamicum WT and ΔsigB. C. glutamicum ΔsigB with empty vector pEPR1 served as a control. The SDs of three measurements are depicted as error bars