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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species

Fig. 4

Graphs showing the number and patterns of the bacterial colonies appearing on the kanamycin-supplemented plates during the deletion of rpoN from E. coli BL21 and upp from B. subtilis 168 using the improved VFAE protocol. a Colonies of E. coli BL21. b Colonies of B. subtilis 168. T-S, single crossover mutant; T-D, double crossover mutant; T-N, wild-type pattern; T-F, failed PCR

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