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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species

Fig. 3

Graphs showing the number and patterns of bacterial colonies generated on the kanamycin-supplemented plate during the deletion of ncRNA31 from P. stutzeri A1501 using the VFAE protocol. a Number of colonies appearing when the VFAE original protocol was applied to the target locus ncRNA31 (represented in three DNA cassettes with different lengths C1–C3). b Number of colonies appearing when the enhanced VFAE protocol was applied to the target locus ncRNA31 (represented in three DNA cassettes C1–C3). T-S, single crossover mutant; T-D, double crossover mutant; T-N, wild-type pattern. T-F, failed PCR

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