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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species

Fig. 2

Cassette construction and colony PCR. a Primer location of the enhanced VFAE protocol. b DNA fragments of the antibiotic cassette and the three different overlapping homologous fragments before ligation. c Three DNA cassettes after ligation. d Ligation product of the VFAE protocol. e Ligation product of the enhanced VFAE protocol. f Colony PCR of the enhanced VFAE protocol; lane 13 shows the wild-type pattern

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