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Table 2 Central composite design matrix of the independent variables with the corresponding experimental values of biomass concentration

From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.

Run

Crude glycerol (x1) (g/L)

Urea (x2) (g/L)

MgSO4 × 7H2O (x3) (g/L)

Biomass (g/L)

1

10

1

0.1

3.27 ± 0.02

2

30

1

0.1

6.99 ± 0.14

3

10

3

0.1

3.16 ± 0.24

4

30

3

0.1

7.89 ± 0.18

5

10

1

0.3

3.36 ± 0.04

6

30

1

0.3

6.83 ± 0.13

7

10

3

0.3

2.98 ± 0.15

8

30

3

0.3

7.47 ± 0.19

9

3.2

2

0.2

1.31 ± 0.03

10

36.8

2

0.2

8.08 ± 0.12

11

20

0.32

0.2

2.61 ± 0.09

12

20

3.68

0.2

5.26 ± 0.1

13

20

2

0.032

5.65 ± 0.15

14

20

2

0.368

6.24 ± 0.29

15

20

2

0.2

6.01 ± 0.12

16

20

2

0.2

5.94 ± 0.07

17

20

2

0.2

6.02 ± 0.11

18

20

2

0.2

6.01 ± 0.09

19

20

2

0.2

5.96 ± 0.15

20

20

2

0.2

6.01 ± 0.06