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Table 2 Central composite design matrix of the independent variables with the corresponding experimental values of biomass concentration

From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.

Run Crude glycerol (x1) (g/L) Urea (x2) (g/L) MgSO4 × 7H2O (x3) (g/L) Biomass (g/L)
1 10 1 0.1 3.27 ± 0.02
2 30 1 0.1 6.99 ± 0.14
3 10 3 0.1 3.16 ± 0.24
4 30 3 0.1 7.89 ± 0.18
5 10 1 0.3 3.36 ± 0.04
6 30 1 0.3 6.83 ± 0.13
7 10 3 0.3 2.98 ± 0.15
8 30 3 0.3 7.47 ± 0.19
9 3.2 2 0.2 1.31 ± 0.03
10 36.8 2 0.2 8.08 ± 0.12
11 20 0.32 0.2 2.61 ± 0.09
12 20 3.68 0.2 5.26 ± 0.1
13 20 2 0.032 5.65 ± 0.15
14 20 2 0.368 6.24 ± 0.29
15 20 2 0.2 6.01 ± 0.12
16 20 2 0.2 5.94 ± 0.07
17 20 2 0.2 6.02 ± 0.11
18 20 2 0.2 6.01 ± 0.09
19 20 2 0.2 5.96 ± 0.15
20 20 2 0.2 6.01 ± 0.06