Table 1 Bacterial strains and plasmids used in this study
From: Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11
Strains and plasmids | Characteristicsa | Source or citation |
|---|---|---|
Lysobacter enzymogenes | ||
OH11 | Wild type, KmR | Qian et al. (2009) CGMCC No. 1978 |
Δlsp1 | lsp1 in-frame deletion mutant, KmR | This study |
Δlsp2 | lsp2 in-frame deletion mutant, KmR | This study |
Δlsp3 | lsp3 in-frame deletion mutant, KmR | This study |
Δlsp1(lsp1) | ∆lsp1 harboring plasmid pBBR-lsp1, GmR, KmR | This study |
Δlsp2(lsp2) | ∆lsp2 harboring plasmid pBBR-lsp2, GmR, KmR | This study |
Δlsp3(lsp3) | ∆lsp3 harboring plasmid pBBR-lsp3, GmR, KmR | This study |
Δlsp1(pBBR) | ∆lsp1 harboring plasmid pBBR1-MCS5, GmR, KmR | This study |
Δlsp2(pBBR) | ∆lsp2 harboring plasmid pBBR1-MCS5, GmR, KmR | This study |
Δlsp3(pBBR) | ∆lsp3 harboring plasmid pBBR1-MCS5, GmR, KmR | This study |
Δlsp12 | The lsp1 & lsp2 deletion mutant, KmR | This study |
Δlsp23 | The lsp2& lsp3 deletion mutant, KmR | This study |
Δlsp13 | The lsp1& lsp3 deletion mutant, KmR | This study |
Δlsp123 | The triple lsp deletion mutant, KmR | This study |
Escherichia coli | ||
DH5α | F−, φ80dlacZΔM15, ∆(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(r −k ,m +k ), phoA, supE44, λ−, thi-1, gyrA96 | Qian et al. (2012) |
Plasmids | ||
 pEX18GM | Suicide vector with a sacB gene, GmR | Hoang et al. (1998) |
 pBBR1-MCS5 | Broad-host-range vector with a P lac promoter | Kovach et al. (1995) |
 pEX18-lsp1 | pEX18GM with two flanking fragments of lsp1, GmR | This study |
 pEX18-lsp2 | pEX18GM with two flanking fragments of lsp2, GmR | This study |
 pEX18-lsp3 | pEX18GM with two flanking fragments of lsp3, GmR | This study |
 pBBR-lsp1 | pBBR1-MCS5 cloned with a 1275-bp fragment containing intact lsp1 and its predicted promoter | This study |
 pBBR-lsp2 | pBBR1-MCS5 cloned with a 1591-bp fragment containing intact lsp2 and its predicted promoter | This study |
 pBBR-lsp3 | pBBR1-MCS5 cloned with a 1655-bp fragment containing intact lsp3 and its predicted promoter | This study |