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Table 1 Bacterial strains and plasmids used in this study

From: Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11

Strains and plasmids Characteristicsa Source or citation
Lysobacter enzymogenes
OH11 Wild type, KmR Qian et al. (2009)
CGMCC No. 1978
Δlsp1 lsp1 in-frame deletion mutant, KmR This study
Δlsp2 lsp2 in-frame deletion mutant, KmR This study
Δlsp3 lsp3 in-frame deletion mutant, KmR This study
Δlsp1(lsp1) lsp1 harboring plasmid pBBR-lsp1, GmR, KmR This study
Δlsp2(lsp2) lsp2 harboring plasmid pBBR-lsp2, GmR, KmR This study
Δlsp3(lsp3) lsp3 harboring plasmid pBBR-lsp3, GmR, KmR This study
Δlsp1(pBBR) lsp1 harboring plasmid pBBR1-MCS5, GmR, KmR This study
Δlsp2(pBBR) lsp2 harboring plasmid pBBR1-MCS5, GmR, KmR This study
Δlsp3(pBBR) lsp3 harboring plasmid pBBR1-MCS5, GmR, KmR This study
Δlsp12 The lsp1 & lsp2 deletion mutant, KmR This study
Δlsp23 The lsp2& lsp3 deletion mutant, KmR This study
Δlsp13 The lsp1& lsp3 deletion mutant, KmR This study
Δlsp123 The triple lsp deletion mutant, KmR This study
Escherichia coli
DH5α F, φ80dlacZΔM15, ∆(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(r k ,m +k ), phoA, supE44, λ, thi-1, gyrA96 Qian et al. (2012)
Plasmids
 pEX18GM Suicide vector with a sacB gene, GmR Hoang et al. (1998)
 pBBR1-MCS5 Broad-host-range vector with a P lac promoter Kovach et al. (1995)
 pEX18-lsp1 pEX18GM with two flanking fragments of lsp1, GmR This study
 pEX18-lsp2 pEX18GM with two flanking fragments of lsp2, GmR This study
 pEX18-lsp3 pEX18GM with two flanking fragments of lsp3, GmR This study
 pBBR-lsp1 pBBR1-MCS5 cloned with a 1275-bp fragment containing intact lsp1 and its predicted promoter This study
 pBBR-lsp2 pBBR1-MCS5 cloned with a 1591-bp fragment containing intact lsp2 and its predicted promoter This study
 pBBR-lsp3 pBBR1-MCS5 cloned with a 1655-bp fragment containing intact lsp3 and its predicted promoter This study
  1. aKmR, GmR = Kanamycin-, Gentamicin-, respectively