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Table 2 Purification of the d-Hyd(co) and d-Carb via functionalized magnetic beads

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Enzyme Fraction A (mU/mL) Avol (mU/mL) Atot (mU) cprot, tot (mg) Aspez (mU/mg) Recovery (%) Purification fold
d-Hyd(co) Crude extract 7.7 15.5 15.5 11.97 1.3
Eluate 1.8 3.7 1.8 0.04 46.4 11.9 35.9
d-Carb Crude extract 76.4 152.9 152.9 15.57 10.0
Eluate 49.7 99.4 49.7 0.04 1243.0 32.5 126.6