Table 2 Purification of the d-Hyd(co) and d-Carb via functionalized magnetic beads
Enzyme | Fraction | A (mU/mL) | Avol (mU/mL) | Atot (mU) | cprot, tot (mg) | Aspez (mU/mg) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|---|---|---|
d-Hyd(co) | Crude extract | 7.7 | 15.5 | 15.5 | 11.97 | 1.3 | – | – |
Eluate | 1.8 | 3.7 | 1.8 | 0.04 | 46.4 | 11.9 | 35.9 | |
d-Carb | Crude extract | 76.4 | 152.9 | 152.9 | 15.57 | 10.0 | – | – |
Eluate | 49.7 | 99.4 | 49.7 | 0.04 | 1243.0 | 32.5 | 126.6 |