Table 3 Analysis of variance (ANOVA) for the quadratic model of ACN content, TEAC and BGS activity
Source | Degree of freedom | Sum of squares | Mean square | f value | p value |
|---|---|---|---|---|---|
Total anthocyanin | |||||
Model | 4 | 908.37 | 227.09 | 66.01 | <0.0001 |
X1 | 1 | 48.02 | 48.02 | 13.96 | 0.0028 |
X2 | 1 | 4.80 | 4.80 | 1.40 | 0.2602 |
X 21 | 1 | 470.18 | 470.18 | 136.66 | <0.0001 |
X 22 | 1 | 338.41 | 338.41 | 98.36 | <0.0001 |
Lack-of-fit | 8 | 26.71 | 3.44 | 0.92 | 0.5776 |
Pure error | 4 | 14.58 | 3.64 | ||
TEAC (antioxidant capacity) | |||||
Model | 7 | 186.90 | 26.70 | 100.02 | <0.0001 |
X1 | 1 | 10.35 | 10.35 | 38.78 | 0.0002 |
X2 | 1 | 2.00 | 2.00 | 7.49 | 0.0230 |
X3 | 1 | 3.25 | 3.25 | 12.18 | 0.0068 |
X 21 | 1 | 89.09 | 89.09 | 333.76 | <0.0001 |
X 22 | 1 | 63.22 | 63.22 | 236.84 | <0.0001 |
X 23 | 1 | 3.04 | 3.04 | 11.40 | 0.0082 |
X1X3 | 1 | 3.24 | 3.24 | 12.14 | 0.0069 |
Lack-of-fit | 5 | 0.70 | 0.14 | 0.33 | 0.8720 |
Pure error | 4 | 1.70 | 0.42 | ||
BGS activity (%) | |||||
Model | 6 | 451.25 | 75.21 | 106.65 | <0.0001 |
X1 | 1 | 8.40 | 8.40 | 11.92 | 0.0062 |
X2 | 1 | 0.14 | 0.14 | 0.19 | 0.6708 |
X3 | 1 | 2.60 | 2.60 | 3.69 | 0.0838 |
X 21 | 1 | 198.43 | 198.43 | 281.39 | <0.0001 |
X 22 | 1 | 174.63 | 174.63 | 247.63 | <0.0001 |
X 23 | 1 | 27.81 | 27.81 | 39.44 | <0.0001 |
Lack-of-fit | 6 | 0.000 | 0.000 | 0.000 | 1.0000 |
Pure error | 4 | 7.05 | 1.76 | ||