Table 1 Primers used in this study
Primer name | Sequence (5′–3′) |
|---|---|
FeaB-XbaI | AACTCTAGATTTAAGAAGGAGATATAATGACAGAGCCGCATGTAGCAG |
FeaB-NheI–BamHI | ACAGGATCCGCTAGCTTAATACCGTACACACACCGACTTAGTTT |
AldB-XbaI | ATCTCTAGATTTAAGAAGGAGATATAATGACCAATAATCCCCCTTCAGC |
AldB-SacI–BamHI | TGTGAGCTCGGATCCTCAGAACAGCCCCAACGGTT |
AldH-XbaI | ATCTCTAGATTTAAGAAGGAGATATAATGAATTTTCATCATCTGGCTTACTG |
AldH-NheI–BamHI | TCAGGATCCGCTAGCTCAGGCCTCCAGGCTTATCC |
ARO8-XbaI | AACTCTAGATTTAAGAAGGAGATATAATGATGACTTTACCTGAATCAAAAGACTTTTC |
ARO8-XhoI | CCGCTCGAGCTATTTGGAAATACCAAATTCTTCGTATAA |