Table 4 Kinetic parameters of the eight best variants and WT for glucuronic acid including the standard deviation of three measurements
Variant | Specific activity (U/mg) | Km (mM) | Catalytic efficiency | |
|---|---|---|---|---|
(s−1mM−1) | (%) | |||
WT | 391 ± 17 | 0.7 ± 0.1 | 286.4 ± 13.0 | 100 |
H236K | 234 ± 5 | 1.3 ± 0.1 | 97.5 ± 2.2 | 34.0 |
A41P/H236K | 407 ± 8 | 1.2 ± 0.0 | 179.5 ± 3.5 | 62.7 |
H101Y/H236K | 218 ± 6 | 1.0 ± 0.1 | 116.7 ± 3.4 | 40.8 |
H101N/H236K | 228 ± 5 | 0.9 ± 0.1 | 139.7 ± 3.2 | 48.8 |
A41P/H101Y/H236K | 280 ± 6 | 0.9 ± 0.1 | 167.3 ± 3.6 | 58.4 |
A41P/H101Y/H236R | 302 ± 4 | 1.0 ± 0.1 | 160.5 ± 2.0 | 56.0 |
A41P/H101N/H236K | 248 ± 4 | 0.9 ± 0.1 | 149.7 ± 2.25 | 52.3 |
A41P/H101N/H236R | 330 ± 3 | 1.0 ± 0.0 | 170.0 ± 1.7 | 59.3 |