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Table 4 Kinetic parameters of the eight best variants and WT for glucuronic acid including the standard deviation of three measurements

From: Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design

Variant Specific activity (U/mg) Km (mM) Catalytic efficiency
(s−1mM−1) (%)
WT 391 ± 17 0.7 ± 0.1 286.4 ± 13.0 100
H236K 234 ± 5 1.3 ± 0.1 97.5 ± 2.2 34.0
A41P/H236K 407 ± 8 1.2 ± 0.0 179.5 ± 3.5 62.7
H101Y/H236K 218 ± 6 1.0 ± 0.1 116.7 ± 3.4 40.8
H101N/H236K 228 ± 5 0.9 ± 0.1 139.7 ± 3.2 48.8
A41P/H101Y/H236K 280 ± 6 0.9 ± 0.1 167.3 ± 3.6 58.4
A41P/H101Y/H236R 302 ± 4 1.0 ± 0.1 160.5 ± 2.0 56.0
A41P/H101N/H236K 248 ± 4 0.9 ± 0.1 149.7 ± 2.25 52.3
A41P/H101N/H236R 330 ± 3 1.0 ± 0.0 170.0 ± 1.7 59.3