Table 1 Details of the PCR primers used in this study
Name | Amplicon | Sequence | Reference |
|---|---|---|---|
GlC1 179R | phaC1 | 5′-AAGGTCAACGCCCTGCTGGGT-3′ 5′-GGTGTTGTCGTTGTTCCAGTAGAGGATGTC-3′ | (Ciesielski et al. 2010) (Solaiman et al. 2000) |
ZRT5 ZRT3 | phaZ | 5′-GAAGTCATCGCCTTTGATGTGCC-3′ 5′-ATCATCCACAGCACCTTGGGCTTG-3′ | This study This study |
C2RT5 C2RT3 | phaC2 | 5′-GCGGCGTGGCTCACCTG-3′ 5′-GAAGCTGTTGGTCGCGCTG-3′ | This study This study |
D-RTf D-RTr | phaD | 5′-CATCAGCCCAGGCAACCTGTAC-3′ 5′-GCGCTCGACGATCAAGTGCAG-3′ | This study This study |
F-RTf F-RTr | phaF | 5′-GTCATGTTTAGACGGAATACCCAG-3′ 5′-GCGGCCAACCACCAGCTTG-3′ | This study This study |
I-RTf I-RTr | phaI | 5′-GCACCGGTCAGCTTCTCGATC-3′ 5′-GGAGCGAACTTGAAGAAGCC-3′ | This study This study |
phaG2F phaG2R | phaG | 5′-TTCAAACGCTTCAACTACCGCC-3′ 5′-CGGTCTTGTTCTCCATGTCCAG-3′ | This study This study |
341F 515R | 16S rRNA | 5′-CCT ACG GGA GGC AGC AG-3′ 5′-AAT CCG CGG CTG GCA-3′ | (López-Gutiérrez et al. 2004) |