Fig. 1
From: Cloning and characterization of the Type I Baeyer–Villiger monooxygenase from Leptospira biflexa
Sequence homology analysis of BVMOLepto. a Maximum-likelihood phylogenetic tree of recombinant BVMOs. The scale bars indicate the number of substitutions per site per unit of branch length. The aLRT values are shown at the nodes: >0.75 (black) and <0.75 (grey). The colors of branches indicate the groups I (pink), II (orange), III (blue), IV (red), V (cyan), VI (violet) and VII (gray) as previously defined (Ferroni et al. 2014; Szolkowy et al. 2009). Blue circles indicate recombinant BVMOs that were insoluble or their activity was not detected. BVMO from L. biflexa is indicated in bold. Protein sequences with their corresponding accession numbers are listed in Additional file 1: Table S1. b Multiple sequence alignment of six representative BVMOs belonging to different clades. The partial alignment of PAMO from T. fusca (Q47PU3), CHMO from Acinetobacter sp. NCIMB 9871 (BAA86293), HAPMO from P. fluorescens ACB (AAK54073), CPMO from Comamonas sp. NCIMB 9872 (BAC22652), CDMO from R. ruber SC1 (AAL14233) and BVMO from L. biflexa (ABZ97795) is shown. The two Rossmann-fold motifs (GxGxxG/A) and the two consensus sequences of Type I BVMOs (G/AGxWxxxxF/YPG/MxxxD and FxGxxxHxxxWP/D) are written in bold